DAF-12 regulates pathogenic defense.

<p>(<b>A</b>) Survival curve of <i>daf-12</i> RNAi-treated worms exposed to <i>P. aeruginosa</i> (PA) relative to control RNAi-treated worms (<i>P</i><0.0001). (<b>B</b>) <i>P. aeruginosa</i> killing assay of wild-type N2, <i>daf-12</i>(<i>sa156</i>) (<i>P</i><0.001) and <i>daf-12</i> (<i>rh61rh411</i>) (<i>P</i><0.001) worms. (<b>C</b>) Lifespan curve of N2, <i>daf-12</i>(<i>sa156</i>) (<i>P</i> = 0.4118) and <i>daf-12</i>(<i>rh61rh411</i>) (<i>P</i> = 0.0019) worms. (<b>D</b>) Transmission electron micrographs (TEM) of a gut section from wild-type N2 and <i>daf-12(sa156)</i> worms fed <i>E. coli</i> or <i>P. aeruginosa</i> for 48 hours. Red arrows indicate microvillis. (Scale bars 1000 nm) All data shown above are representative of at least three independent experiments (n≥50 adult nematodes per strain).