Effects of culture with FCS on PSAF/HeLa-R cells.

<p><b>A: Representative microphotographs of the CPE on PSAF/HeLa-R cells</b> Left panel shows the PSAF/HeLa-R cells maintained in MEM with 10% CS (62 days p.i.). Right panel shows the PSAF/HeLa-R cells maintained in DMEM with 10% FCS for 12 days (from 50 days to 62 days p.i.). CPE on the PSAF/HeLa-R cells significantly increased by the culture with FCS for 12 days. Magnification: ×100. <b>B: Representative microphotographs of the immunocytochemistry</b> Left 6 panels show the PSAF/HeLa-R cells maintained in MEM with 10% CS. Right 2 panels show the PSAF/HeLa-R cells maintained in DMEM with 10% FCS. Upper and lower panels show Nomarski and fluorescent images, respectively. Virus antigen was detected with anti-SAFV-3 antiserum pre-absorbed by the homogenates of HeLa-R cells and Alexa Fluor 594-conjugated anti-rabbit IgG antibody. Viral antigen positive cells were shown in a part of PSAF/HeLa-R cells cultured with CS. After cultivation with FCS for 12 days, however, viral antigen positive cells clearly increased. Magnification: x400.