Viral protein expression in pDC and monocytes after co-culture with VRP-infected cells.

<p>Enriched pDC were either infected with CSFV, VRPΔE^rns or co-cultured with CSFV- or VRPΔE^rns-infected SK-6 cells for 20 h, and then analyzed by three-color FCM to determine the NS3 expression in pDC (defined as CD4⁺CD172a^low, left side dot plots) and monocytes (defined as CD4⁻CD172a^high, right side dot plots). For the CD4/NS3 and CD172a/NS3 dot plots, the blue dots represent NS3 staining of infected cultures overlaid on red dots representing the NS3 staining on non-infected cultures. The percentage of NS3 cells is shown in red for the infected cultures. On the left side the mean IFN-α values and viral titers measured in the cultures are shown. The results are representative of triplicate cultures repeated in three independent experiments.