Effects of MF on CaV1.2 and IP3R.

A: Immunofluorescence staining of CaV1.2 subunit of LTCC in FL cells. The groups were treated as described in Fig 1 and in Materials and Methods. The horizontal line represents 20 μm; average gray value of CaV1.2 per cell in at least 50 cells in each condition was quantified in the histogram; p-value > 0.05 when compared with Sham. B: Western blot assay (left) of p-CaV1.2 (top lane) and p-IP3R (middle lane) in FL cells; Sham: sham-exposed; MF: exposed to 0.4 mT MF for 30 min; EGF: treated with 100 nM EGF for 30 min; NM: pretreated with 0.02 mM NIF for 40 min, then exposed to 0.4 mT 50 Hz MF for 30 min. Relative gray value / content of the proteins to the Sham groups after normalized with the GAPDH was shown in the histogram (right). *: p-value S1 Table.