Effect of MF (2 MT, 50 Hz) on VEGFR expression and activation.

<p>Protein of equal quantity was separated by 8% SDS-page and electroblotted on to a PVDF membrane. Representative blots show effects exerted by MF (2 mT, 50 Hz) on VEGFR2 total expression and phosphorylation after 1 h, 6 h and 12 h of exposure A). Histograms show values obtained by means ± SD of relative protein bands calculated in 3 experiments after incubation with anti-VEGFR2 antibody B) and with anti-pVEGFR2 antibody C). All protein bands were verified using an anti-tubulin antibody. Asterisks indicate statistical differences * P<0.05 D) Immunofluorescence of HUVECs exposed (bottom panels) or not (upper panels to MF for 1 h, 6 h and 12 h. Phosphor-VEGFR2 receptors were stained with a secondary antibody FITC-conjugated. Images shows that after MF exposure, the density of the phosphorylated receptors resulted decreased at each time point investigated.