Culture of cPGCs in serum-containing or chemically defined media.

(A) Images of cPGCs cultured in a serum-containing (FAcs) and chemically defined (FAot or FAits) media for 24, 96, and 168 hr. cPGCs were seeded at a density of 1 × 104 cells/mL. Scale bar: 100 μm. (B) Fold increase in the total number of cPGCs after culture in each type of media for 24, 96, and 168 hr. Cell proliferation was assessed using the CCK-8 assay. Data are mean ± SEM, the statistical significance of difference among three groups was indicated. **** p < 0.0001. (C) Flow cytometric data. The number indicates the percentage of cells stained with an anti-SSEA-1 antibody (green). Isotype staining was performed as a control (white). (D) Percentages of SSEA-1+ cPGCs in FAcs and FAot media. Data are mean ± SEM. NS, not significant.