CTGF promoter activity is inhibited when neurons are co-cultured with GBM cells.

<p>Graph shows Firefly/Renilla luciferase activity of GBM95 or U87 cells transfected with CTGF-lux and Renilla plasmids co-cultured with different amounts of freshly dissociated neurons. Note that 5×10⁴ neurons were able to inhibit more than 40% of luciferase activity (comparing first to third bar, p<0.05) and 2×10⁵ neurons were able to inhibit more than 50% of luciferase activity controlled by the CTGF promoter (A) (comparing first to fourth bar, p<0.01). As a positive control 0.5 nM of TGFβ-1 was used and we could observed an increase of almost 40% of luciferase activity (comparing first to fifth bar, p<0.05). 1×10⁵ P0 neurons were able to inhibit almost 40% of luciferase activity when co-cultured with U87 cells (B) (p<0.05).