Downregulation of CTGF inhibits U87 cell migration.

<p>Representative images of HE stained migrated cells. U87 cells (A) U87 platted with P0 neurons (B) Graph showing the percentage of cells that migrate in each condition (C). U87 were transfected with control siRNA (D) or siRNA to down regulate CTGF expression (E). Statistical analysis of the effects with P0 neurons and downregulation of CTGF on the GBM02 migration (F). Downregulation of CTGF dramatically reduced U87 cell migration in vitro. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055605#s3" target="_blank">Results</a> are mean±SD from three experiments each performed in duplicate. *p<0.05 and **p<0.01, statistically significant difference.