Downregulation of CTGF inhibits GBM95 cell migration.

<p>Representative images of HE stained migrated cells. GBM95 cells (A) GBM95 platted with P0 neurons (B) Graph showing the percentage of cells that migrate in each condition (C). GBM95 transfected with control siRNA (D) or siRNA to downregulate CTGF expression (E). Statistical analysis of the effects of downregulation of CTGF on the GBM95 migration (F). Transwell migration of co-cultures of GBM95 with P0 neurons and GBM95 siRNA to downregulate CTGF shows a significant reduction in motility when CTGF levels are reduced. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055605#s3" target="_blank">Results</a> are mean±SD from three experiments each performed in duplicate. The numbers of invasive cells were significant different from the control group (*p<0.05 and **p<0.01).