Inhibition of CTGF in GBM cells by P0 neurons do not require TGFβ/SMAD2/3 signaling.

<p>(A) Western blot analysis of the cell extracts from GBM95, GBM02 or U87 and GBM95, GBM02 or U87 co-cultured with P0 neurons. From top to bottom, the pictures show membranes reacted with anti-phospho SMAD2/3 and anti-α tubulin antibodies. Molecular weight in kDa is shown on the left side of the picture. (B–D) Graphs show Firefly/Renilla luciferase activity of GBM95 (B), GBM02 (C) or U87 (D) cells transfected with 3TP-lux and Renilla plasmids were cultured alone or P0 neurons, in presence or absence of TGFβ-1 (1 nM). Each point represents the average of three independent experiments done in triplicate.*p<0.05 (in comparison to the control).