Tol-DCs have a reduced capacity to stimulate T lymphocytes.

<p>(<b>A</b>) DCs were cultured with allogeneic PBL at different ratio (1∶20 or 1∶100) for seven days. Upper-left panel data represent the mean ± SD of a representative experiment carried out in triplicate of the seven (upper-right graph) that were independently performed. (<b>B</b>) Antigen-specific T-cell responses. CD4⁺ T cells we cultured with autologous DCs pre-loaded with the superantigen TSST-1 (left graph) or with tetanus toxoid (+ presence and – absence of TT) at a 1∶20 ratio for seven days. T-cell proliferation was determined in triplicate by ³H thymidine incorporation. Data represent the mean ± SD of n = 3 independently performed experiments. Student’s <i>t</i>-test: *<i>p</i><0.05, **<i>p</i><0.001.