TSLP alters naïve CD4 T cell to express Th2 cytokines both on its effect on DCs and T cells.

<p>(A and B) DO11.10 CD4 T cells were cocultured with wild type <i>BMDCs</i> (A) were incubated plus ova peptides (10 ug/ml) and +/− TSLP <i>(15 </i><i>ng/ml)</i>. Supernatants were collected after 48 hrs and assayed for cytokines by Bioplex. (C) DO11.10 CD4 T cells were cultured in Th2 biased conditions for 8 days, and restimulated with ova peptides +/− TSLP for 48 hrs and supernatant were assayed for cytokines by Bioplex. (D) BMDCs or (E) lung CD11c cells were incubated with ova peptides (10 ug/ml) along with either presence or absence of TSLP (15 ng/ml) overnight. Next day DCs were washed extensively and CD4 T cells from DO11.10 spleens were added into DC cultures. After 48 hrs, supernatants were assayed for cytokines by Bioplex. Data represent mean ± SEM from 3 repeated experiments with triplicate wells.