<p>(<b>A</b>) and (<b>B</b>): secretion of IL-2 (left) and IFN-γ (right) after 1 day co-culture of CD8+ OT-I T-cells with DCs or B-cells pre-pulsed or not with Ova peptide (10 pg–10 µg/ml). *p<0.01; **p<0.001, unpaired <i>t</i>-test. Bars indicate mean ± s.e.m. Data are representative of three independent experiments.</p
<p>B- and T cell cultures and subsequent intracellular staining were performed as explained in the l...
<p>The spleens were dissected and pooled on day 35 from groups of four mice immunized with vaccine c...
<p>(<b>A</b>) Bar graphs showing the IFN-γ secretion level (pg/mL), as determined by ELISA, in 48-hr...
<p>Levels of (A) IL-10 and (B) IFNγ in DC: T cell co-culture supernatants. T cells were co-cultured ...
<p>De-iniDC clone #1 or BM-DCs were incubated with OVA (13.5 µg/mL) for 24 hours prior to co-culture...
<p>DC were cultured alone (DC) or with live (zVAD treated) or apoptotic (γ-irradiated) B16 cells for...
<p>Transgenic OVA-specific CD8<sup>+</sup> T cells (A-i) and OVA-specific CD4<sup>+</sup> T cells (B...
<p>Mean ± SD of triplicate cultures of CFSE reduction correlates to proliferation of OT.1 CD8<sup>+<...
<p>Naïve CD4+ T cells from control (empty bars) or SLP76-S376A mice (filled bars) were stimulated by...
<p>CD8<sup>+</sup> T cells and γ/δ T cells of the same donors were either mock electroporated, elect...
<p>Pure B cells and T cells were isolated from whole PBMCs through magnetic negative selection. Puri...
<p>Supernatants of 5 day co-cultures of PBMC from the same donor as the NP cells <b>(donor)</b> or f...
<p>(<b>A</b>) Percentage of IFNγ, MIP-1β and IL-4 producing T-cells in uninfected (1<sup>st</sup> ba...
<p>DCs were stimulated with either BbC50sn (100 µg/ml) or LPS (50 ng/ml) or Zymosan (25 µg/ml) for 2...
<p>PBMC from volunteer CVD4000#63 were co-cultured with DC alone (media), or pre-mixed with live or ...
<p>B- and T cell cultures and subsequent intracellular staining were performed as explained in the l...
<p>The spleens were dissected and pooled on day 35 from groups of four mice immunized with vaccine c...
<p>(<b>A</b>) Bar graphs showing the IFN-γ secretion level (pg/mL), as determined by ELISA, in 48-hr...
<p>Levels of (A) IL-10 and (B) IFNγ in DC: T cell co-culture supernatants. T cells were co-cultured ...
<p>De-iniDC clone #1 or BM-DCs were incubated with OVA (13.5 µg/mL) for 24 hours prior to co-culture...
<p>DC were cultured alone (DC) or with live (zVAD treated) or apoptotic (γ-irradiated) B16 cells for...
<p>Transgenic OVA-specific CD8<sup>+</sup> T cells (A-i) and OVA-specific CD4<sup>+</sup> T cells (B...
<p>Mean ± SD of triplicate cultures of CFSE reduction correlates to proliferation of OT.1 CD8<sup>+<...
<p>Naïve CD4+ T cells from control (empty bars) or SLP76-S376A mice (filled bars) were stimulated by...
<p>CD8<sup>+</sup> T cells and γ/δ T cells of the same donors were either mock electroporated, elect...
<p>Pure B cells and T cells were isolated from whole PBMCs through magnetic negative selection. Puri...
<p>Supernatants of 5 day co-cultures of PBMC from the same donor as the NP cells <b>(donor)</b> or f...
<p>(<b>A</b>) Percentage of IFNγ, MIP-1β and IL-4 producing T-cells in uninfected (1<sup>st</sup> ba...
<p>DCs were stimulated with either BbC50sn (100 µg/ml) or LPS (50 ng/ml) or Zymosan (25 µg/ml) for 2...
<p>PBMC from volunteer CVD4000#63 were co-cultured with DC alone (media), or pre-mixed with live or ...
<p>B- and T cell cultures and subsequent intracellular staining were performed as explained in the l...
<p>The spleens were dissected and pooled on day 35 from groups of four mice immunized with vaccine c...
<p>(<b>A</b>) Bar graphs showing the IFN-γ secretion level (pg/mL), as determined by ELISA, in 48-hr...