DC cytokine profile after culture with live or apoptotic B16 cells.

<p>DC were cultured alone (DC) or with live (zVAD treated) or apoptotic (γ-irradiated) B16 cells for 24 h and stimulated or not with LPS and IFNγ. As controls, B16 zVAD and B16γ cells were also cultured alone. DC were then assessed for their ability to produce IL-12p70 (A) or IL-10 (B) by ELISA. In the upper panels, data from one out of three independent experiments are shown. In the bottom panels, for LPS and IFNγ stimulation, relative IL-12p70 and IL-10 productions are expressed as a percentage of the cytokine production obtained for DC alone. Mean percentage values±SEM are shown. The significance of differences between series of results was assessed using a paired t test (n = 3, 3 independent experiments).