<p>DCs were stimulated with either BbC50sn (100 µg/ml) or LPS (50 ng/ml) or Zymosan (25 µg/ml) for 2 days. IL-12 (A) or IL-10 (B). Synthesis were analyzed by ELISA in the supernatant of these cultures after 48 h of stimulation. The results are expressed in picograms per milliliter: each point represents the values of one donor for each stimulation and the heavy bar the mean of cytokine production for each stimulus. Statistical analysis was performed using the Wilcoxon test for paired non-parametric data. Significance is indicated by p value.</p
<p>Freshly isolated peripheral blood mononuclear cells (PBMC) from ten donors were left unstimulated...
<p>PBMCs (2x10<sup>6</sup>cells/well) were stimulated with individually peptides (5 μg/ml). At day 3...
<p>Cells were stimulated for 72 hours with the indicted concentration of peptide and cytokine concen...
<p>DC were cultured alone (DC) or with live (zVAD treated) or apoptotic (γ-irradiated) B16 cells for...
<p>(A) Real-Time PCR was used to assess IL-12p35, IL-12p40 and IL-10 mRNA on single cell levels befo...
<p>After 12 h exposure to ManLAM and/or PIM (at the concentration of 10 µg/ml and 5 µg/ml, respectiv...
<p>DCs were used in the same conditions as in <a href="http://www.plosone.org/article/info:doi/10.13...
<p>The concentrations of (A) IL-6, (B) TNF-α, (C) IL-12 and (D) IL-10 were determined by ELISA assay...
<p>The concentrations of (A) IL-6, (B) TNF-a, (C) IL-12 and (D) IL-10 were determined by ELISA assay...
<p>(A) DC membrane molecule expression at 6 (dotted line) and 24 hours (black line) after LPS or LPS...
<p>(A) IL-6, (B) IL-17, (C) IFN-γ, (D) IL-2 and (E) TNF-α levels measured by ELISA in supernatant fr...
<p>WT, MyD88<sup>−/−</sup>, and TLR2<sup>−/−</sup> DCs (10<sup>6</sup> cells/ml) were stimulated by ...
<p>DCs were stimulated with LPS (100 ng/ml) in the presence of vehicle or CBG for 24 hours. Supernat...
<p>BDCs were stimulated 24 h with LPS (1 µg/ml), poly I∶C (10 µg/ml), imiquimod (10 µg/ml), class A ...
<p>MDDC were either untreated (none) or treated with BpWT or BpCyaA−, the latter in the presence or ...
<p>Freshly isolated peripheral blood mononuclear cells (PBMC) from ten donors were left unstimulated...
<p>PBMCs (2x10<sup>6</sup>cells/well) were stimulated with individually peptides (5 μg/ml). At day 3...
<p>Cells were stimulated for 72 hours with the indicted concentration of peptide and cytokine concen...
<p>DC were cultured alone (DC) or with live (zVAD treated) or apoptotic (γ-irradiated) B16 cells for...
<p>(A) Real-Time PCR was used to assess IL-12p35, IL-12p40 and IL-10 mRNA on single cell levels befo...
<p>After 12 h exposure to ManLAM and/or PIM (at the concentration of 10 µg/ml and 5 µg/ml, respectiv...
<p>DCs were used in the same conditions as in <a href="http://www.plosone.org/article/info:doi/10.13...
<p>The concentrations of (A) IL-6, (B) TNF-α, (C) IL-12 and (D) IL-10 were determined by ELISA assay...
<p>The concentrations of (A) IL-6, (B) TNF-a, (C) IL-12 and (D) IL-10 were determined by ELISA assay...
<p>(A) DC membrane molecule expression at 6 (dotted line) and 24 hours (black line) after LPS or LPS...
<p>(A) IL-6, (B) IL-17, (C) IFN-γ, (D) IL-2 and (E) TNF-α levels measured by ELISA in supernatant fr...
<p>WT, MyD88<sup>−/−</sup>, and TLR2<sup>−/−</sup> DCs (10<sup>6</sup> cells/ml) were stimulated by ...
<p>DCs were stimulated with LPS (100 ng/ml) in the presence of vehicle or CBG for 24 hours. Supernat...
<p>BDCs were stimulated 24 h with LPS (1 µg/ml), poly I∶C (10 µg/ml), imiquimod (10 µg/ml), class A ...
<p>MDDC were either untreated (none) or treated with BpWT or BpCyaA−, the latter in the presence or ...
<p>Freshly isolated peripheral blood mononuclear cells (PBMC) from ten donors were left unstimulated...
<p>PBMCs (2x10<sup>6</sup>cells/well) were stimulated with individually peptides (5 μg/ml). At day 3...
<p>Cells were stimulated for 72 hours with the indicted concentration of peptide and cytokine concen...