Cytokine production by activated adult and neonatal BDCs.

<p>BDCs were stimulated 24 h with LPS (1 µg/ml), poly I∶C (10 µg/ml), imiquimod (10 µg/ml), class A or class C CpG ODN (5 µg/ml), then the supernatants were harvested and the concentration of the different cytokines determined by ELISA. The upper panel shows the results for neonatal BDCs and the bottom panel the ones for adult BDCs. We show here the concentration of TNF-α (A, D), IL-12p40 (B, E) and IFN-α (C, F) in the supernatants from stimulated (black boxes) or non-stimulated (white boxes) BDCs. Results show the mean cytokine production in pg/ml ± SD from 5 or 6 animals. (*<i>p<0.05</i>, Wilcoxon signed rank test).