Effect of <i>Cs</i>TPs-stimulated BMDCs on the regulation of Th2 polarization.

<p>(A) Representative images of IL-4 or IFN-γ positive CD4⁺ T cells after co-culturation with <i>Cs</i>TPs-stimulated BMDCs in the presence of LPS analyzed by FACS. BMDCs were stimulated as described for 24 hours and 1×10⁴ pre-treated BMDCs were co-cultured with 1×10⁵ MACS-sorted CD4⁺ T cells for 10 d. T cells were intracellularly stained for IL-4 and IFN-γ after the stimulation of primed T cells with 1×Cell Stimulation Cocktail (plus protein transport inhibitors) for 5h, and then the stained cells were measured by FACS. (B) Statistical analysis of the ratio of IL-4 positive to IFN-γ positive CD4⁺ T cells. Three representative experiments were shown. Data are presented as mean ± SD and statistical significance was analyzed by one-sided paired Student’s <i>t</i>-test (*, <i>P</i>< 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001 vs. LPS group).