Mature DC cannot substitute for immature DC at either stimulation time points.

<p>Immature lowGM-DC or mature highGM/4-DC were generated to stimulate allogeneic naïve T cells. After 5 days the T cells were restimulated with the same type of DC or in an opposite setting as indicated in the legend. After another 3 days the T cells were restimulated with the indicated reagents for 3 days before [³H]-thymidine incorporation was detected to measure proliferation. The data from one experiment shown are representative of 3 independent experiments. Values represent the mean±standard deviation error bars of triplicate cultures from one experiment.