Knockdown of p14^ARF reversed the suppression of cell migration and invasiveness in CDK5RAP3 knockdown HCC cells.

<p>(<b>a</b>) The CDK5RAP3 stable knockdown SMMC-7721 cells were transfected with p14^ARF or control siRNA. <i>Top,</i> Western blotting showing p14^ARF knockdown in cells; <i>bottom,</i> The bar chart showed the quantitation of migrated cells in three independent experiment (*, <i>P</i> = 0.005, Student’s <i>t</i>-test). Representative photomicrographs were shown. (<b>b</b>) Similar to <b>a</b>), but invasion assay were performed. The bar chart showed the quantitation of the invaded cells in three independent experiment (*, <i>P</i> = 0.05, Student’s <i>t</i>-test).