Regulation of p14^ARF localization and protein expression by CDK5RAP3.

<p>(<b>a</b>) The p14^ARF and CDK5RAP3 protein levels in stable CDK5RAP3 knockdown SMMC-7721 clones (shCDK5RAP3#1 and #2), vector control and parental cells <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042210#pone.0042210-Mak1" target="_blank">[5]</a> were compared by Western blotting using indicated antibodies, respectively. (<b>b</b>) The stable CDK5RAP3 knockdown SMMC-7721 (shCDK5RAP3#2) and vector control cells were treated with 100 µg/ml cycloheximide or DMSO (vehicle), and harvested at the indicated time points. p14^ARF and <i>β</i>-actin protein levels were determined by Western blotting. V: DMSO treatment. <i>Top</i>: Western blotting; <i>bottom</i>: quantification of p14^ARF protein level.