TRPV5 knockdown reverses the effect of VDR knockdown on caki-1 cell proliferation, migration and invasion.

<p>(A) TRPV5 mRNA expression was detected by RT-PCR and was normalized to that of β-actin. TRPV5 protein expression was detected by WB using β-actin as a loading control. shV+T is shVDR+shTRPV5 co-treated. (B) Cell proliferation ability was analysed by the MTT assay in control, shVDR-treated, or shVDR+shTRPV5-treated caki-1 cells; (C-D) Cell migration and invasion ability were measured by the transwell assay. The graph indicates the mean ± SD of the number of cells from 5 random high-power fields (magnification, ×200). *p <0.05, **p <0.01.