(A) Tumor cells were transfected with siRNAs and Rac activation was quantitated

Closed bars, BE; open bars, MDA-MB-231; shaded bars, SNB19 (mean + SEM; ≥ 4). *, P < 0.05; **, P < 0.01; unpaired Student's test. (B) BE (closed bars) and MDA-MB-231 cells (open bars) were transfected with siRNAs and assayed for collagen-I invasion (mean + SEM; ≥ 4). *, P < 0.05; **, P < 0.01; unpaired Student's test. (C) BE cells were seeded for invasion assays in serum-free DME or DME + 5% serum and either control IgG or antivitronectin antibody (mean + SEM; ≥ 4). **, P < 0.01; unpaired Student's test. (D) HEK 293T cells were transfected with siRNAs, and after 48 h, transfected with uPAR or empty vector. Rac activity was measured 24 h later. (E) Quantitation of Rac-activation assays in HEK 293T cells (mean + SEM; ≥ 5). *, P < 0.05; unpaired Student's test versus nontargeting. Immunoblots showing knockdown are in Fig. S2 (C and D), available at .<p><b>Copyright information:</b></p><p>Taken from "uPAR promotes formation of the p130Cas–Crk complex to activate Rac through DOCK180"</p><p></p><p>The Journal of Cell Biology 2008;182(4):777-790.</p><p>Published online 25 Aug 2008</p><p>PMCID:PMC2518715.</p><p>