Tc_REG suppress mature osteoclasts by effecting the cytoskeletal reorganization:

<p>Osteoclasts were cultured either alone or with Tc_REG on bovine bone chips for 24 hrs. T-cells were then removed, and osteoclasts were stained with phalloidin-Texas Red to visualize actin rings. Representative images are shown in panel <b>A</b>. Quantitation of three independent experiments is shown in panel <b>B</b>. Panel <b>C</b> is quantitation of phalloidin staining of osteoclasts plated on tissue culture treated dishes. Statistical significance of actin ring area was assessed by non-parametric paired T test: **: P<0.01 in comparison to osteoclast alone.