Osteoclast differentiation of RAW264.7 cells induced by RANKL.

<p>RAW264.7 cells were induced to differentiate into osteoclasts as described in the Materials and Methods section, for 1, 4 and 7 days. (A, B, and C) Morphological changes in the cells were observed under a light microscope (original magnification 100x). (D) Representative images of TRAP staining (original magnification 100x). Bars: 50 μm.