Cytoskeletal reorganization, lysosomal acidification and MARCKS phosphorylation in PKC-δ KO osteoclasts.

<p>(A) Osteoclasts on bone slices were immunofluorescently stained with Rhodamine Phalloidin, anti-α-tubulin, and Hoechst to visualize F-actin, α-tubulin and DNA by confocal microscopy. Scale bar represents 100 μm. (B) Osteoclasts were treated with Acridine Orange, which displays green fluorescence at neutral pH. Acridine Orange green fluorescence intensity was measured in a fluorescence microplate reader. Bar charts represent mean ± standard deviation. Experiments were performed in triplicate. n.s., no significance (p-value>0.05). (C) Osteoclasts on bone slices were immunofluorescently stained pMARCKS, Rhodamine Phalloidin and DAPI to examine the phosphorylation levels of MARCKS by confocal microscopy. Scale bar represents 20 μm.