CLSM images showing the distribution of ClC-7, V-ATPase and F-actin in osteoclasts.

<p>A, B: In osteoclasts incubated on bone (A) or glass coverslips (B), ClC-7 (green) shows a clear circular distribution. C–F: In osteoclasts incubated on bone, ClC-7 (red) (C) is restricted to a circular strip immediately inside the F-actin ring (D) and immediately outside the central, V-ATPase-rich area (green) (E), and does not co-localize with either V-ATPase or F-actin (F). A, B, D, counterstained with DAPI to show nuclei. Bars 10 µm (A–C) and 50 µm (D).