CD68 can be found in a dome-like pattern in osteoclasts cultured on bovine cortical bone slices.

<p>(A) BMMs were seeded onto bone slices with 44 ng/mL M-CSF and 100 ng/mL RANKL and differentiated into osteoclasts over 4 days. Cells were fixed with 4% paraformaldehyde/PBS and stained with Alexa-488-conjugated phalloidin (actin, green), Hoescht (nuclei, blue), and either anti-CD68 (CD68, red) or rat non-immune IgG_2a (IgG_2a Merge) antibody followed by Alexa-647-conjugated anti-Rat IgG. Scale bars for CD68 staining are 40μm; scale bar for IgG_2a staining is 20μm. Images are representative of 3 independent experiments. (B) Enlarged merge image from A. * and + indicate corresponding XZ and YZ cross sectional images, respectively. Scale bar is 40μm. Image is representative of 3 independent experiments. (C) 3-D reconstruction of osteoclast cross sectioned in B with actin in green, nuclei in blue, and CD68 in red.