The cell phenotype of BrdU labeled surviving cells in the DG of WT and G93A mice subject to treadmill running (EX) or sedentary lifestyle (SED).

<p>(A) Representative confocal microscopic images of triple fluorescent staining in the DG of the hippocampus in WT mice. Green: BrdU, red: NeuN, Blue: GFAP. (B) A representative confocal microscopic image showed two newly generated neurons (white arrows) colocalized with BrdU and NeuN in WT mice. (C) A representative confocal microscopic image showed one newly generated astrocyte (yellow arrow) colocalized with BrdU and GFAP in WT mice. (D) The percentage of double labelled BrdU and NeuN cells was 22.4% higher in G93A-SED than in WT-SED collapsed across sex. *G93A-SED>WT-SED, P = 0.007, n = 5–6 per group. (E)There was no significant difference in the percentage of BrdU and NeuN double labelled cells between WT-EX and WT-SED mice, n = 5–6 per group. (F) There was no significant difference in the percentage of BrdU and NeuN double labelled cells between G93A-EX and G93A-SED mice, n = 5–6 per group. Data are means ± SEM. Scale bar = 100 µm in A; 5 µm in B and C.