The effect of modulation of VILIP-1-expression and cAMP-signaling on Snail1 mRNA levels.

<p><b>A</b>. RT-PCR analysis showing the suppression of Snail1 mRNA by ectopic expression of VILIP-1 in VILIP-1-negative, aggressive CC4A and CH72T3 cells (lanes 2, 3 and 5, 6) and the blocking of this effect by application of DDA (500 µM) (lanes 3, 6). siRNA knock down of VILIP-1 in VILIP-1-positive less aggressive CC4B and CH72 cells caused no alteration of the expression level of Snail1 (lanes 8, 10). <b>B</b>. Densitometry of RT-PCR analysis in A, lanes 1 to 6. Intensity of Snail1 bands was normalized to the intensity of GAPDH control PCR bands (CC4A+VILIP-1: p = 0.035, CH72T3+VILIP-1 p = 0.037). Bars represent the mean of three experiments. Error bars indicate standard deviations. Asterisks indicate the level of significance.