Snail1 contributes to the down-regulation of pro-apoptotic genes induced by TGF-β1.

<p>(A) 603B cells were treated with TGF-β1 for indicated periods of time. Alterations of mRNA expression for pro-apoptotic genes Pten, Bim, Bax, Bid and Puma were assessed by qRT-PCR. (B) 603B cells stably expressing control shRNA or Snail1 shRNA were treated with TGF-β1 for 6 days. Changes of pro-apoptotic gene mRNA levels were determined by qRT-PCR. Data represent an average of three independent experiments. Values are means ± SE. *<i>p</i><0.05 compared to non-TGF-β1 treated cells; ^#<i>p</i><0.05 compared to shRNA-NS control. shRNA-NS = non specific shRNA control; Snail1-KD = snail shRNA knockdown.