Resveratrol reduces Cisplatin-induced Snail expression.

<p>Western Blot analysis of EMT markers Snail, Vimentin and ZEB1 in A2780 and A2780CP (A) cells in response to 10 µm Cisplatin with or without increasing doses of Resveratrol. Densitometric analysis is shown under each cell line Western Blots. Resveratrol inhibits Cisplatin-induced Snail expression in a dose-dependent manner but slightly increases ZEB1 and Vimentin levels. The Resveratrol dose-dependent decrease in P-Erk2 levels suggests the involvement of the Erk pathway in Resveratrol-induced Snail downregulation. The EMT markers Snail, Vimentin and ZEB1 expression were analysed by RT-PCR in A2780 and A2780CP (B) cells treated with 10 µm Cisplatin with or without increasing doses of Resveratrol. Resveratrol or Cisplatin treatment had no effect on ZEB1 and Vimentin mRNA levels. Vimentin and ZEB1 densitometry is shown under each cell line. Snail mRNA expression was decreased only in A2780 cells, suggesting another mechanism could be involved in A2780CP cells. Images shown are representative of three independent experiments. In graphs, columns identified with different letters are significantly different (p<0.05). In graphs, mock: untreated cells, R40: Resveratrol 40 µM, CP: Cisplatin, R20CP: Resv. 20 µM + Cisplatin, R40CP: Resv. 40 µM + Cisplatin, R60CP: Resv. 60 µM + Cisplatin.