Expression levels of Snail1 mRNA in VILIP-1-positive and VILIP-1-negative SCC and effect of EGF.

<p><b>A</b>. RT-PCR analysis showing the expression of Snail1 in VILIP-1-negative, aggressive CC4A and CH72T3 cells using GAPDH mRNA expression as internal control. <b>B</b>. Effect of EGF stimulation (lanes 2, 3 and 5, 6) and of additional forskolin treatment (lanes 3 and 6) on the expression of Snail1 mRNA in VILIP-1-positive, less aggressive CC4B and CH72 cells. For comparison the expression of VILIP-1 mRNA and GAPDH mRNA is shown. Representative pictures of three independent experiments are shown. <b>C</b>. Densitometry of RT-PCR analysis in B, lanes 1 to 6. Intensity of Snail1 bands was normalized to the intensity of GAPDH control PCR bands (CC4B+EGF: p = 0.039, CH72+EGF p = 0.029, CC4B+EGF+FSK: p = 0.04, CH72+EGF+FSK: p = 0.047). Bars represent the mean of three experiments. Error bars indicate standard deviations. Asterisks indicate the level of significance.