Loss of Snail1 failed to impede TGF-beta induced alterations of morphology and gene expression (A) Representative images of cell morphology of wild-type (WT) RMG (I, II) and Snail1 KO1 (III, IV) cells, after incubation for 72 h in the absence (I, III) or presence (II, IV) of TGF-beta (10 ng/mL).

<p>Scale bar indicates 100 μm. Photos were taken of 5–10 images per sample. Experiments were repeated three times. (B) Quantitative RT-PCR assessment of the cells shown in (A) for the indicated genes. Values are expressed as the mean ± s.e.m. of triplicate samples. Experiments were repeated twice. Statistical significance is indicated by an asterisk (*<i>P</i> < 0.05 and ** <i>P</i> < 0.01 using Students’ <i>t</i>-test). (C) Western blot analysis of the cells shown in (A) for the indicated proteins. Vinculin was used as a loading control. Experiments were repeated twice.