VLP-induced modulation in transcription profile of genes involved in signaling of innate immune response in 293 cells by PCR Array.

<p>293 cells were grown overnight in 60 mm dishes and were infected with 10 µg of purified VLPs suspended in OPTI-MEM (Invitrogen). Mock infected cells served as negative control. The inoculum was adsorbed on the cell monolayers for 3 hours at 37°C when it was supplemented with fresh OPTI-MEM and further incubated overnight when total cell RNA was extracted according to the manufacturer's (SA Biosciences) instructions. <b>A</b>: shows the integrity of the RNA used for this analysis. Note the ∼2∶1 ratio of 28S:18S which is a good indication of the integrity of the RNA. Equal concentration of the RNA from the mock and VLP-exposed cells was used for expression profiling by RT² PCR Profiler PCR Array according the manufacturer's (SABiosciences) instructions. <b>B</b>: Shows the heat map, it is a visual illustration of the relative expression levels in the VLP-stimulated vs. the “mock” stimulated control cells of the all the genes in the array: The four genes differentially expressed by a factor of ∼4 fold or greater (shown as red squares) are listed below the heat map.