NJ001 induced apoptosis of SPC-A1 cells.

<p>SPC-A1 cells were cultured with or without 200 µg/mL NJ001 or MCA2849 for 24 h and 48 h. (A) Morphological changes in SPC-A1 cells were observed under inverted microscope (×100). a, 24 h NJ001; b, 24 h MCA2849; c, 24 h McAb free; d, 48 h NJ001; e, 48 h MCA2849; f, 48 h McAb free. (B) Apoptosis was analyzed by flow cytometry. (C) Each column and error bar represents the mean ± SD of three independent experiments (**<i>P</i><0.001). The amount of late apoptosis was determined as the percentage of Annexin V⁺/PI⁺ cells.