Apoptotic changes measured by flow cytometry.

<p>The first set of experiments included four groups: KTC-1 cells received no treatment (<b>A</b>), 20 MBq/ml ¹³¹I (<b>B</b>), 5 µmol/L Bay 11-7082 (<b>C</b>) or combination (<b>D</b>) for 24 hours. Then cells were harvested by trypsinization, double stained by FITC-conjugated annexin V and propidium iodide, and then subjected for flow cytometry. In the second set of experiments, KTC-1 cells were first treated with no oligonucleotide control (<b>E</b>), scrambled oligonucleotides (<b>F</b>) or p65 siRNA transfection (<b>G</b>). Then these three groups of cells were exposed to ¹³¹I (20 MBq/ml) for 24 hours (<b>H–J</b>). And flow cytometry was conducted. Similar results were obtained in three independent experiments.