Flow cytometry analysis of liver cell apoptosis.

<p>(A) Flow cytometry analysis of the selected cells; (B) negative control from the TUNEL kit; (C) positive control from the TUNEL kit; (D) cell apoptosis in the control group; (E) 50 μg/kg MC-LR treated group at 12 h; (F) Percentage of apoptotic cells, n = 4. Asterisk indicates the statistical difference relative to the control. M1 represents normal cells <i>vs</i>. the total number of cells, M2 represents the apoptotic cells <i>vs</i>. the total number of cells. Duncan’s test was used to determine the significant difference between groups. The same letter indicates no significant difference between groups, while different letters indicate a significant difference between groups with <i>p</i> < 0.05.