Analysis of apoptosis and cell cycle arrest in U937 cells by flow cytometry.

<p>Cells were treated with 150 μg/ml of pLLD and binding of Annexin-V/FITC to phosphatidyl serine was measured by flow cytometry to determine the percentages of apoptotic cells in time dependent manner (A). Study of cell cycle arrest in U937 cells was carried out by propidium iodide staining. Percentage of G0/G1 cell population increases after treatment of 150 μg/ml of pLLD in time dependent manner (B). The data are represented as mean ± SEM from triplicate independent experiments (*P>0.05; ** P>0.01).