β,β-Dimethylacrylshikonin induced apoptosis in SGC-7901 cells.

<p>(A) Cell morphology was detected by using an invert microscope with 200×magnification. (B) DNA condensation (white arrow) was measured by DAPI stain and analyzed under a fluorescent microscope with 200×magnification. (C) The apoptotic status was evaluated by Annexin V-FITC binding assay. The lower right part (Annexin V-FITC⁺/PI⁻) was considered as early stage of apoptotic cells and top right part (Annexin V-FITC⁺/PI⁺) was considered as late stage of apoptotic cells. The lower left part (Annexin V-FITC⁻/PI⁻) was considered as viable cells and the upper left part (Annexin V-FITC⁻/PI⁺) was considered as necrotic cells.