DH281, DH285 and DH287 induce apoptosis at different concentrations.

<p>DH281, DH285 and DH287 induce apoptosis at different concentrations. HeLa or MRC5 cells were incubated in the presence of a compound at 0, 0.21, 1.05, and 5.25 µM for 48 hr. After harvest, the cells were stained with FITC-conjugated Annexin V and PI and then subjected to FACS analysis. PI⁺FITC<b>⁻</b> represents dead cells; FITC⁺PI⁺ represents late apoptotic cells; FITC<b>⁻</b>PI<b>⁻</b> represents live cells; PI<b>⁻</b>FITC⁺ represents early apoptotic cells. The apoptotic cells are the combination of cells with PI⁺FITC⁺ and PI<b>⁻</b>FITC⁺.