eNOS Ser¹¹⁷⁷ phosphorylation and NO production induced by acute treatment with sildenafil.

<p><b>A</b>: Western Blot analysis of eNOS Ser¹¹⁷⁷ phosphorylation. Means ± S.E. (n = 4). * <i>p</i><0.05 vs Ctrl; ° <i>p</i><0.05 vs insulin; **<i>p</i><0.01 vs sildenafil; °°<i>p</i><0.05 vs sildenafil+insulin. <b>B</b>: Sildenafil treatment (1 µM/5 h) induces NO production that is partially inhibited from LY. Means ± S.E. (n = 6). *<i>p</i><0.05 <i>vs</i> Ctrl; **<i>p</i><0.01 <i>vs</i> Ctrl; ‡ <i>p</i><0.05 <i>vs</i> insulin; ¶ <i>p</i><0.05 <i>vs</i> LY; ¶¶ <i>p</i><0.05 <i>vs</i> LY+insulin; # <i>p</i><0.05 and ## <i>p</i><0.01 <i>vs</i> sildenafil; ∥ <i>p</i><0.05 <i>vs</i> sildenafil+insulin. <b>C</b>: L-NAME effects on NO production induced by sildenafil treatment. L-NAME reduces NO production induced by acute treatment with sildenafil. Means ± S.E. (n = 6). *p<0.05 <i>vs</i> Ctrl; # <i>p</i><0.05 <i>vs</i> sildenafil. Full bar represent conditions without sildenafil, dashed bars represent sildenafil treatment.