<p>Sildenafil chronic treatment (1 µM/72 h) enhances eNOS phosphorylation in basal condition and after HG treatment, but not significantly after Gluc-N treatment. Full bars represent conditions without sildenafil, dashed bars represent sildenafil treatment. Ctrl was expressed as 100%. Means ± S.E. (n = 5). *<i>p</i><0.05 <i>vs</i> Ctrl, ‡ <i>p</i><0.05 <i>vs</i> insulin; † <i>p</i><0.05 <i>vs</i> HG; †† <i>p</i><0.05 <i>vs</i> HG + insulin.</p
<p>(a) Representative immunoblots of <i>p</i>-eNOS (Ser1177/Thr495/Ser114), total eNOS, nNOS and iNO...
<p>Panel A: U-II (10 µM) caused an increase in eNOS phosphorylation expressed as p-eNOS/eNOS ratio i...
<p>(A) Relative amount of iNOS protein expression after MB treatment. (B) Relative amount of iNOS pr...
<p><b>A</b>: Western Blot analysis of eNOS Ser<sup>1177</sup> phosphorylation. Means ± S.E. (n = 4)....
<p>Sildenafil treatment induces NO production in insulin resistance condition (HG and Gluc-N) in HUV...
.Human umbilical vein endothelial cells (HUVECs) were treated with insulin in presence of glucose 30...
<p><b>(A)</b> Influence of 1-NAME on the effect of 1 nM nifedipine on HG (22 mM glucose)-induced inc...
Background: The efficacy of Phosphodiesterase 5 (PDE5) inhibitors to re-establish endothelial functi...
BACKGROUND: The efficacy of Phosphodiesterase 5 (PDE5) inhibitors to re-establish endothelial functi...
<p>(A) Western blotting of eNOS, sGCα1 and sGCβ1, (B) densitometric analysis of sGCα1/β-actin, (C) d...
<p>Western blots for phosphor- (p-) and total- (t-) ERK1/2 in the RV (A) and the LV (B). Quantificat...
The efficacy of Phosphodiesterase 5 (PDE5) inhibitors to re-establish endothelial function is reduce...
<p>The endothelial cells were treated with high glucose (HG) for 72 h, and then treated with L-NAME ...
<p><b>A</b>. HUVECs were incubated with the vehicle (DMSO) or telmisartan for 2 h after pretreatment...
<p>(A) Representative immunoblots of <i>p</i>-eNOS (Ser1177/Thr495/Ser114/Ser633) and total eNOS in ...
<p>(a) Representative immunoblots of <i>p</i>-eNOS (Ser1177/Thr495/Ser114), total eNOS, nNOS and iNO...
<p>Panel A: U-II (10 µM) caused an increase in eNOS phosphorylation expressed as p-eNOS/eNOS ratio i...
<p>(A) Relative amount of iNOS protein expression after MB treatment. (B) Relative amount of iNOS pr...
<p><b>A</b>: Western Blot analysis of eNOS Ser<sup>1177</sup> phosphorylation. Means ± S.E. (n = 4)....
<p>Sildenafil treatment induces NO production in insulin resistance condition (HG and Gluc-N) in HUV...
.Human umbilical vein endothelial cells (HUVECs) were treated with insulin in presence of glucose 30...
<p><b>(A)</b> Influence of 1-NAME on the effect of 1 nM nifedipine on HG (22 mM glucose)-induced inc...
Background: The efficacy of Phosphodiesterase 5 (PDE5) inhibitors to re-establish endothelial functi...
BACKGROUND: The efficacy of Phosphodiesterase 5 (PDE5) inhibitors to re-establish endothelial functi...
<p>(A) Western blotting of eNOS, sGCα1 and sGCβ1, (B) densitometric analysis of sGCα1/β-actin, (C) d...
<p>Western blots for phosphor- (p-) and total- (t-) ERK1/2 in the RV (A) and the LV (B). Quantificat...
The efficacy of Phosphodiesterase 5 (PDE5) inhibitors to re-establish endothelial function is reduce...
<p>The endothelial cells were treated with high glucose (HG) for 72 h, and then treated with L-NAME ...
<p><b>A</b>. HUVECs were incubated with the vehicle (DMSO) or telmisartan for 2 h after pretreatment...
<p>(A) Representative immunoblots of <i>p</i>-eNOS (Ser1177/Thr495/Ser114/Ser633) and total eNOS in ...
<p>(a) Representative immunoblots of <i>p</i>-eNOS (Ser1177/Thr495/Ser114), total eNOS, nNOS and iNO...
<p>Panel A: U-II (10 µM) caused an increase in eNOS phosphorylation expressed as p-eNOS/eNOS ratio i...
<p>(A) Relative amount of iNOS protein expression after MB treatment. (B) Relative amount of iNOS pr...