Reduced ROS production and eNOS uncoupling in the penis of Sickle mice by sildenafil treatment.

<p>ROS production was measured by luminol activity (a; n = 4–6) and nitrotyrosine activity (b; n = 7). Representative Western blots (c) and densitometric analysis (d; n = 5) of eNOS dimers and monomers in the penis after low-temperature SDS-PAGE. eNOS is uncoupled in the penis of Sickle mice compared to WT mice, while continuous treatment of Sickle mice with sildenafil citrate recouples eNOS. The results are expressed as dimer/monomer ratio. Each bar represents the mean ± SEM using ANOVA post-hoc Tukey test. *<i>P</i><0.05 vs. WT; **<i>P</i><0.05 vs. Sickle.