Evidence of enhanced eNOS-catalyzed NO formation induced by the 2K1C operation.

<p><b>A</b>, Western blot detection of p-eNOS (serine 1177) and eNOS in 50 µg kidney homogenates of sham- and 2K1C-operated WT mice and quantification with respect to the ratio of p-eNOS to eNOS in kidneys of sham-operated WT (n=8 and 7 mice, respectively). * P< 0.05 versus WT-sham, unpaired Student's t test. <b>B</b>, concentration-response curves for angiotensin II-induced vasoconstriction of isolated perfused kidneys of sham- and 2K1C-operated WT mice in the presence of L-NAME (n=9 and 6 mice, respectively). * P< 0.05, and ** P< 0.01 versus WT-sham, unpaired Student's t test. <b>C</b>, cyclic GMP content determined in renal cortical slices of sham- and 2K1C-operated WT mice without any addition (15 slices of 5 mice per group) or in the presence of sildenafil (100 µM, 10 min; 15 slices of 5 mice per group). ***P< 0.001 versus WT-sham, unpaired Student's t test. Experiments were performed using the non-clipped kidney of 2K1C mice and the respective kidney of sham mice.