Tau phosphorylation in mouse hippocampal tissue 30 min following the administration of propofol under hypothermic (<i>A</i>) or normothermic (<i>B</i>) conditions.

<p>Hippocampal protein extracts were separated by SDS-PAGE and levels of tau phosphorylation were determined using antibodies directed at the AT8 (Ser²⁰²/Thr²⁰⁵;<i>A1,B1</i>), CP13 (Ser²⁰²;<i>A2,B2</i>), and PHF-1 (Ser³⁹⁶/Ser⁴⁰⁴;<i>A3,B3</i>) phosphoepitopes, or total tau (<i>A4, B4</i>). Relative immunoreactive band intensities are expressed as a percent of control (Ctl; intralipid) and are displayed for each phosphoepitope and total tau. For each condition, 2 representative data are displayed with Ctl (n = 4), and Prop (n = 5). Data are expressed as mean ± SD. *** denotes <i>P</i><0.001, ** denotes <i>P</i><0.01, and * denotes <i>P</i><0.05 vs. Ctl with unpaired <i>t</i>-test.