Effect of propofol on tau phosphorylation in Tau-SH-SY5Y cells.

<p>Cells were harvested after 1 h incubation at 37°C (n = 4) or 30°C (n = 3) in the absence of propofol (<i>A</i>), or following 1 h exposure to 10% intralipid in DMEM (Ctl, n = 3) or propofol (Prop, n = 5) at 3 µg/ml (16.8 µM), both at 37°C (<i>B</i>). Cell lysate protein extracts were separated by SDS-PAGE and the level of tau phosphorylation was determined using antibodies directed at the AT8 (<i>A1, B1</i>), CP13 (<i>B2</i>), or PHF-1 (<i>B3</i>) phosphoepitopes, or total tau (<i>A2, B4</i>). Relative immunoreactive band intensities are expressed as a percent of control and are displayed for each phosphoepitope and total tau. Tau phosphoepitopes are normalized on total tau. For each condition, 2 representative data are displayed. Data are expressed as mean ± SD, with *, **, and *** denoting <i>P</i><0.05, <i>P</i><0.01 and <i>P</i><0.001 vs. ctl, respectively with unpaired <i>t</i>-test.