The impact of propofol on phosphorylation of p38 and the release of IL-1β, IL-6 and TNF-α under LPS stimulation after inhibition of Annexin A1 in THP-1.

<p>A. Lysis samples of THP-1 cells were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis of Annexin A1. B. Gray intensity analysis of the western blot results of six groups. Each bar represents the mean ± S.D. No significant differences were found between L' group and L'+P' Group (p>0.05). L group: LPS group with wide-type Annexin A1; L+P group: LPS+propofol group with wide-type Annexin A1 ; L' group: LPS group with transfected shRNA of Annexin A1; L'+P' group: LPS+propofol group with transfected shRNA of Annexin A1. C. Lysis samples of THP-1 cells were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis of phosphorylation of p38. D. Gray intensity analysis of the western blot results of four groups. Each bar represents the mean ± S.D. No significant differences were found between L' group and L'+P' Group (p>0.05). L group: LPS group with wide-type Annexin A1; L+P group: LPS+propofol group with wide-type Annexin A1 ; L' group: LPS group with transfected shRNA of Annexin A1; L'+P' group: LPS+propofol group with transfected shRNA of Annexin A1. E. the experession of mRNA of TNF-α IL-1β, IL-6 in THP-1 cells. Each bar represents the mean ± S.D, no significant differences were found L'+P' group compared with L' group.