Effect of propofol on tau kinases in mouse cortical tissue 0.5 h and 2 h following the administration of propofol under normothermic conditions.

<p>Cortical protein extracts were separated by SDS-PAGE and levels of kinases were determined using antibodies directed at activated or total kinases as follow: (<i>1</i>) GSK-3β phospho-S9, (<i>2</i>) total GSK-3 (α and β), (<i>3</i>) phospho-ERK, (<i>4</i>) total ERK, (<i>5</i>) phospho-JNK, (<i>6</i>) total JNK, (<i>7</i>) phospho-CaMKII, (<i>8</i>) total CaMKII, (<i>9</i>) phospho-AKT, (<i>10</i>) total AKT, (<i>11</i>) CDK5, and (<i>12</i>) P35. Relative immunoreactive band intensities are expressed as a percent of control (Ctl; intralipid) and are displayed for each epitope. For each condition, 1 representative datum is displayed with Ctl (n = 6), 0.5 h (n = 6), and 2 h (n = 7). Data are expressed as mean ± SD. *, ** and *** denote <i>P</i><0.05, <i>P</i><0.01 and <i>P</i><0.001 vs. ctl, respectively; ANOVA with Newman-Keuls <i>post hoc</i> test.