Tau phosphorylation in mouse hippocampal tissue 1 h following the administration of DMSO at 0, 1, 2, and 4 ml/kg i.p.

<p>Hippocampal protein extracts were separated by SDS-PAGE and levels of tau phosphorylation were determined using antibodies directed at the (A) AT8 (pSer²⁰²/pThr²⁰⁵), (B) PHF-1 (pSer³⁹⁶/pSer⁴⁰⁴), (C) AT180 (pThr²³¹/pSer²³⁵) phophoepitopes, or (D) total Tau. For each phosphoepitope, relative immunoreactive band intensities are expressed as a ratio to total tau and are displayed as a percentage of control (0 ml/kg of DMSO). For each condition, 2 representative data are displayed with controls (0 ml/kg; n = 5), 1 ml/kg (n = 5), 2 ml/kg (n = 5) and 4 ml/kg (n = 5). Data are expressed as mean ± SD. *, **, *** denote <i>p</i><0.05, <i>p</i><0.01 and <i>p</i><0.001 versus control, respectively; ANOVA with Newman-Keuls <i>post hoc</i> test.