Relative number of infectious virus particles added to different cell lines to achieve MOI 3 for each virus/cell combination.

<p>Most experiments in this study were conducted using 24-well tissue culture plates and nearly 100% confluent cells treated with RBV in SFM (or mock-treated with SFM) and infected with VSV-GFP or SeV-GFP (or mock-infected with SFM) at MOI of 3 CIU/cell of the tested cell line. The MOI of 3 CIU/cell for each virus/cell type combination was calculated by infecting each cell line with serial dilutions of VSV-GFP or SeV-GFP virus stock in SFM and counting infectious foci with the aid of fluorescence microscopy (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011265#pone-0011265-g007" target="_blank">Figure 7A</a>). VSV CIU^BHK – number of cell infectious units (infectious particles) determined by titration of VSV-GFP virus stock on BHK21 cells. SeV CIU^HeLa - number of cell infectious units calculated by titration of SeV-GFP virus stock on HeLa cells.